The basis of AKLIDES® platform technology is the AKLIDES® Kyto that analyses cell-based immunofluorescence assays for autoimmune diagnostics. Due to its especially developed software solution the system is one of a kind.
It is the first time that an algorithm-based software is used for analyzing complex biological cell structures and tissues for medical routine diagnostics. The automatic image storage and analysis of immunofluorescence provides the opportunity of impartial analysis as well as archiving, export option and the installation of a patient database that comprises result protocols and immunofluorescent pictures. It is therefore possible to access and compare results in the database at anytime.
AKLIDES® Kyto at a glance
Fully automated screening and evaluation of cells
Analysis in < 1 minute per well
Pattern specific quantification
Archiving of results and database
Sample-specific report in pdf. and csv. format
Intelligent live image mode
Cell based Immunfluorescence tests
Immunofluorescence (IF) is an immunohistochemical method for biomolecule detection using antigens or antibodies labelled by fluorescent dyes. Especially indirect immunofluorescence (IIF) is used to detect circulating autoantibodies (AAb) in patient sera associated with autoimmune diseases. To do so, a fixed biological test system (consisting of tissue or cells) is brought into contact with a patient´s serum. Autoantibodies from serum interact specifically with target antigens (e.g. proteins) within tissue or cells. A secondary fluorochrome labelled antibody binds to the AAb subsequently.
Excitation energy provided by a light source generates a fluorescence signal that visualizes the AAb indirectly on the target tissue or cells and creates a specific fluorescence pattern.
Substrate for cell based IIF
Generally three different cell substrates are routinely employed in autoimmune
• HEp-2 cell line for the detection of anti-nuclear antibodies (ANA)
• The hemoflagellate Crithidia luciliae for the detection of AAb to dsDNA and the
diagnosis of systemic lupus erythematodes (SLE)
• Human granulocytes to proof systemic vasculitis due to AAb targeting the antigenic
structures in the cytoplasm of neutrophil cells (ANCA)
• Tissue section
Advantages of cell based IIF Disadvantages of cell based IIF
‣ Simple and short test procedure ‣ Subjective analysis
‣ Low costs for reagents ‣ No complete automatisation
‣ Multiparametric assay ‣ Insufficient standardisation
‣ Archiving not possible
AKLIDES® resolves the disadvantages: